I have been using the large expression dataset from RNAseq and was thinking about any possible effects that the passage number at which the cell line was sampled for sequencing might have on the expression. I was wondering whether anywhere in the metadata for cell lines which have been sequenced, there would be information on at which passage number they were sequenced?
Hi, unfortunately we don’t record this information anywhere. Most of our cell lines are historical, and have gone through many passages over years. Unfortunately I don’t think there would be any way to track this information.
OK, thanks for your help!