User drug sensitivity (PRISM Repurposing Primary screen)

Q&A
1

Hello,

What do the values in this mean? Log2 ic50?

2

We generally have the units at the bottom of plots, which should answer the question. The primary screen was a single point assay, so it should be some measure of viability normalized to a control.

Pulling up an arbitrary compound in data explorer, it appears it’s log2 fold change over the control.

image

Thanks,
Phil

3

Thank you for your reply. Does “a single pont” mean a certain concentration? Cells were treated at a certain concentration, and cell viability was normalize to control?

4

Correct. The primary repurposing screen was done at a single concentration, and cell viability was normalized to a control. You can read more details about the experiment by following the references listed on this page which describes the repurposing dataset and associated paper.