Prism Repurposing Primary Screen

Are the values for the PRISM Repurposing Primary Screen IC50 values? Do we know how many data points were used and what formula was used to calculate this value?

Hi Giuliana,

The primary screen consists of only a single dose (2.5 uM), compounds are screened at three replicates and the reported values are the log2-fold change viability values between the treatment and the negative control (DMSO).

For the details, you can check the methods part of Discovering the anticancer potential of non-oncology drugs by systematic viability profiling | Nature Cancer



Many thanks.