Hi, I have a question about interpreting gene effect in terms of selection of genes that are ‘hits’.
Is using gene effect for a specific cell line sufficient to do this? In other words, can I select a threshold for a negative gene effect and call genes that score below that threshold as hits in my cell line of interest? If so, what would you recommend as the best approach to selecting the threshold?
I can see that the skewed-LRT data isn’t publicly available therefore I’m trying to figure out whether using gene effect to select gene hits is appropriate.