Recently a reviewer from one of our submitted manuscripts called our attention pointing at the big discrepancy between the expression levels of our target protein in an specific AML cell line detected by Western Blotting, compare to the expression levels of the same protein in the same cell line shown in DEPMap PROTEOMICS (Q9UPP1). Using Western Blotting we have very low protein expression close to zero compare with other AML cell lines whereas DepMap shows in their PROTEOMICS (Q9UPP1) a similar high expression level of our target protein in that particular cell line compare with the same other AML cells. Is it a logical way to explain this discrepancy?
many thanks in advance!!