Hello, i am working on validating Drug-target screens to validate the hypothesis that the drug is inhibiting the gene product by examining that the gene ko and the drug have similar signatures (effects). however, i am lost to which dataset is more appropriate for building the correlation model betwe…

We do share a raw log fold change per CRISPR guide, but that data is not ideal to use directly, as there are multiple known experimental artifacts (CN correction and guide efficacy being dominate ones of the top of my head) which should be corrected for to understand the effect of knocking out a gen…

Hi, sorry for interrupting, but how about checking the GDSC dataset?

Hi Song, thank you for the recommendation, but the main bottleneck with the dataset is the limited number of drugs. How about the sanger cell viability, is it comparable to the chronos score for genes?

Sorry, but can you clarify the ‘Sanger cell viability’ with the relevant link? In my opinion, the Chronos score and IC50 (or AUC) values will be difficult to compare directly, as they are computed in different ways and have distinct meanings.

it is called sanger-viability.csv with the following download link: https://depmap.org/portal/data_page/?tab=allData&releasename=Sanger%20GDSC1%20and%20GDSC2&filename=sanger-viability.csv wouldn’t the Chronos and IC50 (or AUC50) be correlated in drug-target pair with similar transcriptomic profile…

Drug-Target validation using CRISPR and PRISM repurposing dataset

Q&A

Mohamed_El_Moussaoui June 29, 2025, 6:10pm 3

thank you for the clarification, is there something similar to chronos but for drugs, like AUC\IC50 of drugs across different cell lines

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Drug-Target validation using CRISPR and PRISM repurposing dataset

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