Culture media info

I am trying to find out more details about the culture conditions that were used in the DepMap screens. The “Achilles_metadata.csv” file is quite helpful, but I have a few follow up questions. It is all based on the additives like glutamine or glutamax or sodium pyruvate or sodium bicarbonate that are expected to be present in the base media.

  1. Is “DMEM + 10% FBS” the same as “DMEM + 10% FBS + 1% Glutamine” or “DMEM + 10% FBS + 2mM Glutamax” or "DMEM + 10% FBS + 2mM Glutamine” ?

  2. Is “IMDM + 10% FBS” the same as “IMDM + 10% FBS + 2mM Glutamine” or "IMDM + 10% FBS + Glutamax”

  3. Is “RPMI +10% FBS” the same as “RPMI + 10% FBS + Pen-Strep-Glut” or “RPMI + 10% FBS + 2mM Glutamine” or “RPMI + 10% FBS + 2mM L-glutamine + 25mM HEPES” or “RPMI + 10% FBS + 2mM Glutamax” or “RPMI + 10% heat inactivated FBS + 300mg/L L-glutamine + 25mM HEPES + 25mM Sodium bicarbonate”

Hi manojit,

Answers in bold.

Is “DMEM + 10% FBS” the same as “DMEM + 10% FBS + 1% Glutamine” or “DMEM + 10% FBS + 2mM Glutamax” or "DMEM + 10% FBS + 2mM Glutamine” ?

Glutamine & Glutamax are used interchangeably, so all the media that include either of those names are identical. Glutamax is a more stable alternative to L-glutamine (so we always use that) and it comes in liquid form, almost always used at 1% of the complete medium. DMEM + 10% FBS does not include that supplement.

Is “IMDM + 10% FBS” the same as “IMDM + 10% FBS + 2mM Glutamine” or "IMDM + 10% FBS + Glutamax”

Similar to the last question, + Glutamine and + Glutamax are the same thing. IMDM + 10% FBS does not include that supplement.

Is “RPMI +10% FBS” the same as “RPMI + 10% FBS + Pen-Strep-Glut” or “RPMI + 10% FBS + 2mM Glutamine” or “RPMI + 10% FBS + 2mM L-glutamine + 25mM HEPES” or “RPMI + 10% FBS + 2mM Glutamax” or “RPMI + 10% heat inactivated FBS + 300mg/L L-glutamine + 25mM HEPES + 25mM Sodium bicarbonate”

Similar again. RPMI + 10% FBS includes no supplements. Pen/Strep is sometimes included in the annotation but GPP hasn’t included antibiotics in our medium for a few years now and I believe DMX also doesn’t either. (L-)Glutamine/Glu/Glut/Glutamax, HEPES, & Sodium bicarb are all different supplements that all come in liquid form and are almost always used at 1%. Heat inactivated FBS (aka hiFBS) is distinct from regular FBS.