Hi! I am working to select a cell line for a functional study, and am looking for a cell line that doesn’t express the target gene, so we can test the effect of mutant genes. Is there a common process that is used for this?
Right now, I am just sorting by expression, filtering for the tissue type we are interested in, CNS/Brain, and checking against the DepMap proteomic screen data for cell lines where it is available. Is this a common approach?