Interpretation of Drug sensitivity AUC and Logfold change value

Dear Depmap team,

I have some questions regarding how to interpret Drug sensitivity AUC and Logfold change value during compound screening on cell lines:

  1. If cell line A has the higher the drug sensitivity AUC than cell line B on compound C, does it mean that cell line A is more sensitive than cell line B when treated with compound C?
  2. If cell line A has positive higher logfold change value than cell line B on compound C, does it mean that cell line A is more resistant than cell line B when treated with compound C? How about if cell line A has negative logfold change, what does it mean again?

Thank you in advance!

Best,
Marissa

Hi Marissa,

Higher AUC implies less sensitivity to the compound, similarly higher LFC also implies less sensitivity.

As a reasonable heuristic, you can consider AUC as the average of 2^LFC (i.e. foldchange viabilities) over different doses, so they are (roughly) increase and decrease together.

Warmly,
Mustafa

Hi Mustafa,
Thank you very much for the answer!
Best,
Marissa

Hi Mustafa,

To follow up the question above, what is the LFC measured by? cell number in treatment group compared to DMSO group?

Thanks,
Marissa