Hello, I’m trying to compare gene expression of two cell lines but an error occurs
This doesn’t happen with groups with more than two cell lines
The two class comparison is performing a t-test between two groups. As a result, we need a sufficient number of samples in each group to be able to make statistical statements about the groups.
The error you’re seeing is likely due to something that’s assuming there are some minimal number of samples (for example, at some point we compute the stddev of each group, and that will fail if we have fewer than 3 samples).
However, that error message isn’t helpful and we should communicate the minimum number of samples that we need if the current selection is insufficient. I’ll file a bug requesting a better error message.
Thanks,
Phil
