Uncorrected files

Good afternoon,

I have a few questions about the available files:

  1. Speaking of “Achilles_gene_effect_uncorrected.csv”, in the description it says that it is “* Post-Chronos *, Raw Chronos data, prior to copy number correction and scaling”, but what corrections are applied before CN correction and scaling resizing?

  2. Are there also uncorrected data for “CRISPR_gene_dependency”, “CRISPR_gene_effect” and “Achilles_gene_dependency”?

Thank you so much in advance.

Hi Chris,

  1. No other corrections are applied, it’s the direct gene effect inferred by Chronos.
  2. the CRISPR… files are generated after running Harmonia to integrate multiple Chronos runs, so all of them use corrected data.