How can I see the change in gene expression for several genes after the KO of one particular Gene

Q&A
1

Hello,

I am currently looking for a way to see the post-perturbational expression of several Genes after the KO of the Arhgap25 in Leukemia cell lines. Is there any possibility to do that and if so it would be very helpful to have a detailed way to do it.

Thanks a lot

Jannik

2

This kind of question comes up from time to time, but the DepMap datasets only contain RNA expression of lines pre-perturbation. Viability is our sole post-perturbation readout at this time.

So, the data you’re interested in hasn’t been collected in this project.

The CMAP project was the largest screen collecting post-perturbation expression responses in a collection of models that I am aware of. I don’t that the specific case you’re interested is represented in that dataset, but post-perturbation expression was their focus so it may be worth looking if you haven’t already.

Thanks,
Phil