Estimation of uncertainty around the dose-response curve

Hi everyone,
I was wondering, how do you estimate the uncertainty around the AUC value for the drug screens?
Some of the cell lines were tested in 2-3 replicates and some in 6, that would influence how certain we are on the curve fit from cell line to cell line. Also those AUCs become less directly comparable in that case.

Would be grateful for any tip!

Best regards,