CRISPRGeneDependency file integrated both Broad and Sanger datasets?

Q&A
1

Hi everyone,

I wanted to confirm whether the CRISPRGeneDependency file integrates both Broad and Sanger CRISPR datasets. The README states:

“This Achilles dataset contains the results of genome-scale CRISPR knockout screens for Achilles (using Avana Cas9 and Humagne-CD Cas12 libraries) and Achilles combined with Sanger’s Project SCORE (KY Cas9 library) screens.”

The wording is a bit confusing since Achilles appears twice. My interpretation is that the Achilles pipeline processes CRISPR knockout screens generated at the Broad Institute (Avana and Humagne-CD libraries) as well as screens from the Sanger Institute Project Score (KY library), and these are jointly integrated using the Chronos model.

Does that sound correct?

Thanks,
Chris

2

Yes, that’s correct. I’ll let folks know about the confusing wording.

Thanks,

Phil

3

Sounds great, thanks for your confirmation!

Best,

Chris