An asymmetric volcano map

[weiyue1]

I divided the cells into two groups for differential gene analysis and this result occurred. What’s going on here? Please!

[dllahr]

I’ve observed the same problem, even more severe - cases where not only is it asymmetric, but there are no positive values at all

No positive values of effect size reported when using "custom analysis" tool

Hello Let me start by saying how great the data explorer is, and how great the custom analysis tool is. I observed occasionally that when using the “custom analysis” tool within the data explorer that none of the results (or very, very few) have positive values. I would expect a much larger number to have somewhat positive values just by chance. I finally have a concrete example I can share that is sufficiently anonymous to reproduce the result. Here’s a screenshot of the results: [image] …