Discretization of RNAseq gene expression

Q&A
1

Hi,

The RNAseq gene expression data provided are continuous values. There is a suggested way for discretize these values into Underexpressed, Normal, Overexpressed?

Thanks,

2

Unfortunately we don’t have a suggested way to do this. We’ve used a threshold of TPM=1 for calling ‘unexpressed’ genes, but other than that we don’t have a standard procedure for discretizing expression levels like this.

3

Thanks so much for the answer!

Kind Regards,
Matías