# Viability test used?

**URL:** <https://forum.depmap.org/t/viability-test-used/3174>\
**Category:** Q&A\
**Tags:** genetic-screens\
**Created:** [April 2, 2024, 5:56pm UTC](https://forum.depmap.org/t/viability-test-used/3174 "2024-04-02T17:56:10Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![Giacomo\_Graziano](https://yyz1.discourse-cdn.com/flex035/user_avatar/forum.depmap.org/giacomo_graziano/32/980_2.png) [@Giacomo\_Graziano](https://forum.depmap.org/u/Giacomo_Graziano)\
**Post date:** [April 2, 2024, 5:56pm UTC](https://forum.depmap.org/t/viability-test-used/3174/1 "2024-04-02T17:56:11Z")

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If I knocked down the “A” gene using technologies such as RNAi, in two cell lines with opposite dependencies for that gene, how can I evaluate the different dependency? Would a difference in cell growth be observable after knockdown of the gene? Furthermore, which viability tests is DepMap based on to evaluate the effect of gene knockdown?

Thanks in advance