# Type of growth media of cell lines

**URL:** <https://forum.depmap.org/t/type-of-growth-media-of-cell-lines/2747>\
**Category:** Q&A\
**Tags:** portal\
**Created:** [August 7, 2023, 12:47am UTC](https://forum.depmap.org/t/type-of-growth-media-of-cell-lines/2747 "2023-08-07T00:47:51Z")\
**Posts on this page:** 4\
**Page:** 1

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**Author:** ![Reza](https://avatars.discourse-cdn.com/v4/letter/r/b4bc9f/32.png) [@Reza](https://forum.depmap.org/u/Reza)\
**Post date:** [August 7, 2023, 12:47am UTC](https://forum.depmap.org/t/type-of-growth-media-of-cell-lines/2747/1 "2023-08-07T00:47:51Z")

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Hi there,  
I found my data for mRNA expression of ELOVL5 in breast cancer cell lines (MCF7, ZR-75-1, T47D). I was wondering if there is any information on the condition of cell line growth including the types of media, and percentage of FBS?  
Were cell lines cultured in FBS or charcoal-stripped FBS?

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**Author:** ![Samantha\_Stokes](https://yyz1.discourse-cdn.com/flex035/user_avatar/forum.depmap.org/samantha_stokes/32/676_2.png) [@Samantha\_Stokes](https://forum.depmap.org/u/Samantha_Stokes)\
**Post date:** [August 14, 2023, 3:49pm UTC](https://forum.depmap.org/t/type-of-growth-media-of-cell-lines/2747/2 "2023-08-14T15:49:13Z")

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Hello,

For these three lines, the media annotations are:  
MCF7 - MF-015-010: EMEM + 10% FBS + 0.01 mg/ml recombinant insulin  
ZR-75-1 - MF-001-001: RPMI + 10% FBS  
T47D - MF-001-045: RPMI + 10% FBS + 2ug/ml Insulin

We have also updated our media annotations in the Models.csv and ModelCondition.csv metadata files to include codes and a description for all cell lines for your reference in the future.

Best,  
Samantha

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**Author:** ![Reza](https://avatars.discourse-cdn.com/v4/letter/r/b4bc9f/32.png) [@Reza](https://forum.depmap.org/u/Reza)\
**Post date:** [August 15, 2023, 12:02am UTC](https://forum.depmap.org/t/type-of-growth-media-of-cell-lines/2747/3 "2023-08-15T00:02:35Z")

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Thank Samantha,  
That was very informative.

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**Author:** ![JulesL](https://avatars.discourse-cdn.com/v4/letter/j/a88e57/32.png) [@JulesL](https://forum.depmap.org/u/JulesL)\
**Post date:** [August 31, 2023, 10:57am UTC](https://forum.depmap.org/t/type-of-growth-media-of-cell-lines/2747/4 "2023-08-31T10:57:39Z")

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Hello,

I am looking for the media used to culture some cell lines I would like to order.  
In the ModelCondition and Media files from the metadata, I am reading : “MEM + 10% FBS” for one cell line and “RPMI + 10% FBS” for the other. But there are many different MEM and RPMI media available, with different compositions. Is the catalog number and provider of these media available somewhere ?

Thanks so much in advance !  
All the best,  
Jules