# The mutation data of EGFR doesn't match with publications

**URL:** <https://forum.depmap.org/t/the-mutation-data-of-egfr-doesnt-match-with-publications/1594>\
**Category:** Report an Issue\
**Created:** [August 3, 2022, 5:25am UTC](https://forum.depmap.org/t/the-mutation-data-of-egfr-doesnt-match-with-publications/1594 "2022-08-03T05:25:15Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![Qiang](https://avatars.discourse-cdn.com/v4/letter/q/c68b51/32.png) [@Qiang](https://forum.depmap.org/u/Qiang)\
**Post date:** [August 3, 2022, 5:25am UTC](https://forum.depmap.org/t/the-mutation-data-of-egfr-doesnt-match-with-publications/1594/1 "2022-08-03T05:25:15Z")

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I’m searching mutations on the exon 20 of EGFR. However, after I downloaded the csv file, I found that the “End Position” seemed not fit the data in [ensembl.org](http://ensembl.org). As the Transcript ends at [55,211,628] in [ensembl.org](http://ensembl.org), but in the csv file, many mutations locate after [55,273,200].  
Also, many publications report that the EGFR exon 20 encode p761D to p775C. I searched in this range in the “Protein Change” column, and found p.I764V, p.S766F, and p.L770L. But the publication (DOI: [10.1016/j.ctrv.2020.102105) reported that the wildtype EGFR should have 764Y, 766M, and 770D.  
I wonder whether I misunderstand the csv file or did I make any mistakes in my research?

Thank you!

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**Author:** ![jkobject](https://yyz1.discourse-cdn.com/flex035/user_avatar/forum.depmap.org/jkobject/32/82_2.png) [@jkobject](https://forum.depmap.org/u/jkobject)\
**Post date:** [August 8, 2022, 5:18pm UTC](https://forum.depmap.org/t/the-mutation-data-of-egfr-doesnt-match-with-publications/1594/2 "2022-08-08T17:18:56Z")

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Hello Qiang,

I think you are overlooking the reference genome’s build version:

 ![Screenshot 2022-08-08 at 13.11.15](https://canada1.discourse-cdn.com/flex035/uploads/depmap/original/1X/06a5c431c7e6ac0474be658a46671234b8733aa3.png)

We are on hg19 for the mutations to match with TCGA’s data.

Best,

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**Author:** ![Qiang](https://avatars.discourse-cdn.com/v4/letter/q/c68b51/32.png) [@Qiang](https://forum.depmap.org/u/Qiang)\
**Post date:** [August 9, 2022, 1:07am UTC](https://forum.depmap.org/t/the-mutation-data-of-egfr-doesnt-match-with-publications/1594/3 "2022-08-09T01:07:27Z")

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Great jkobject!

That’s in my blind zone, you really saved me!

Thanks a lot!