# shRNA and sgRNA sequences

**URL:** <https://forum.depmap.org/t/shrna-and-sgrna-sequences/561>\
**Category:** Q&A\
**Created:** [April 16, 2021, 8:05pm UTC](https://forum.depmap.org/t/shrna-and-sgrna-sequences/561 "2021-04-16T20:05:22Z")\
**Posts on this page:** 4\
**Page:** 1

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**Author:** ![Louatre](https://avatars.discourse-cdn.com/v4/letter/l/a6a055/32.png) [@Louatre](https://forum.depmap.org/u/Louatre)\
**Post date:** [April 16, 2021, 8:05pm UTC](https://forum.depmap.org/t/shrna-and-sgrna-sequences/561/1 "2021-04-16T20:05:23Z")

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Hi Depmap team,

I was wondering if there is any downloadable list or information regarding the shRNA sequences used (or even sgRNA) in the datasets?

Thanks!

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**Author:** ![pmontgom](https://yyz1.discourse-cdn.com/flex035/user_avatar/forum.depmap.org/pmontgom/32/4_2.png) [@pmontgom](https://forum.depmap.org/u/pmontgom)\
**Post date:** [April 20, 2021, 5:59pm UTC](https://forum.depmap.org/t/shrna-and-sgrna-sequences/561/2 "2021-04-20T17:59:43Z")

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For both the RNAi and the CRISPR datasets the Achilles project generated, we provide the shRNA and sgRNA sequences as a download.

For example: [DepMap Data Downloads](https://depmap.org/portal/download/?release=DepMap+Public+21Q1) contains  
Achilles\_guide\_map.csv which contains the sequences for the sgRNAs among a few other columns.

For the RNAi data, [DepMap Data Downloads](https://depmap.org/portal/download/?release=DEMETER2+Data+v6) contains D2\_combined\_hp\_data.csv which has a column for the shRNA sequences.

Thanks,  
Phil

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**Author:** ![Louatre](https://avatars.discourse-cdn.com/v4/letter/l/a6a055/32.png) [@Louatre](https://forum.depmap.org/u/Louatre)\
**Post date:** [April 20, 2021, 6:26pm UTC](https://forum.depmap.org/t/shrna-and-sgrna-sequences/561/3 "2021-04-20T18:26:26Z")

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Hi Phil,

Thanks a lot, that’s very helpful!! I just downloaded the shRNA sequences table. Is there an easy way for me to find the shRNA for my gene of interest? the table doesn’t contain Gene ID information 🙂

Cheers

L

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**Author:** ![pmontgom](https://yyz1.discourse-cdn.com/flex035/user_avatar/forum.depmap.org/pmontgom/32/4_2.png) [@pmontgom](https://forum.depmap.org/u/pmontgom)\
**Post date:** [April 20, 2021, 7:16pm UTC](https://forum.depmap.org/t/shrna-and-sgrna-sequences/561/4 "2021-04-20T19:16:31Z")

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The shRNA → gene mapping is contained in shRNA-mapping.csv in the same DEMETER 2 release.

Thanks,  
Phil