# Reproduce results from "Prioritization of cancer therapeutic targets using CRISPR–Cas9 screens, Fiona M. Behan""

**URL:** <https://forum.depmap.org/t/reproduce-results-from-prioritization-of-cancer-therapeutic-targets-using-crispr-cas9-screens-fiona-m-behan/1599>\
**Category:** Q&A\
**Created:** [August 3, 2022, 1:53pm UTC](https://forum.depmap.org/t/reproduce-results-from-prioritization-of-cancer-therapeutic-targets-using-crispr-cas9-screens-fiona-m-behan/1599 "2022-08-03T13:53:03Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![Nathalie\_Jeanray](https://yyz1.discourse-cdn.com/flex035/user_avatar/forum.depmap.org/nathalie_jeanray/32/546_2.png) [@Nathalie\_Jeanray](https://forum.depmap.org/u/Nathalie_Jeanray)\
**Post date:** [August 3, 2022, 1:53pm UTC](https://forum.depmap.org/t/reproduce-results-from-prioritization-of-cancer-therapeutic-targets-using-crispr-cas9-screens-fiona-m-behan/1599/1 "2022-08-03T13:53:03Z")

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Hello,

I had a look at article “Prioritization of cancer therapeutic targets using CRISPR–Cas9 screens, Fiona M.Behan” ([Prioritization of cancer therapeutic targets using CRISPR–Cas9 screens | Nature](https://doi.org/10.1038/s41586-019-1103-9)), in which, in Supplementary table 2, we can find a tab “a. fitness gene summary”, by tissue, cancer type and cell line, composed of 0 and 1 values.

However, this list is three years old (2019) and the number of tissues, cancer types and genes is quite limited.

So, I’ve dowloaded the 5 CRISPR datasets from DepMap (CRISPR (Project Score, CERES), etc.) with the hope of being able to reconstruct a table similar to the additional data 2, but with up-to-date data.

Could you please tell me if the CRISPR data from DepMap are enough ?  
Do I have to use BAGEL algorithm to transform continous data into binary ones ?  
Does anyone have an idea on the way to process ?

Thanks in advance,

Best regards,  
Nathalie

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**Author:** ![Nathalie\_Jeanray](https://yyz1.discourse-cdn.com/flex035/user_avatar/forum.depmap.org/nathalie_jeanray/32/546_2.png) [@Nathalie\_Jeanray](https://forum.depmap.org/u/Nathalie_Jeanray)\
**Post date:** [August 22, 2022, 2:07pm UTC](https://forum.depmap.org/t/reproduce-results-from-prioritization-of-cancer-therapeutic-targets-using-crispr-cas9-screens-fiona-m-behan/1599/2 "2022-08-22T14:07:51Z")

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Hello,

Is someone able to answer this question please ? 🙂

Best regards,  
Nathalie

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**Author:** ![Joshua\_Dempster](https://yyz1.discourse-cdn.com/flex035/user_avatar/forum.depmap.org/joshua_dempster/32/19_2.png) [@Joshua\_Dempster](https://forum.depmap.org/u/Joshua_Dempster)\
**Post date:** [August 31, 2022, 8:18pm UTC](https://forum.depmap.org/t/reproduce-results-from-prioritization-of-cancer-therapeutic-targets-using-crispr-cas9-screens-fiona-m-behan/1599/3 "2022-08-31T20:18:29Z")

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Hi Nathalie,

I would contact the corresponding author for help reproducing the manuscript. It’s a little outside the support we can provide.

Best,

Josh