# Question regarding CCLE RNAseq dataset

**URL:** <https://forum.depmap.org/t/question-regarding-ccle-rnaseq-dataset/289>\
**Category:** Q&A\
**Created:** [November 15, 2020, 10:15am UTC](https://forum.depmap.org/t/question-regarding-ccle-rnaseq-dataset/289 "2020-11-15T10:15:30Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![hjook1990](https://avatars.discourse-cdn.com/v4/letter/h/90db22/32.png) [@hjook1990](https://forum.depmap.org/u/hjook1990)\
**Post date:** [November 15, 2020, 10:15am UTC](https://forum.depmap.org/t/question-regarding-ccle-rnaseq-dataset/289/1 "2020-11-15T10:15:30Z")

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Dear DepMap team,

Could I ask what is the relative threshold set against in the CCLE\_RNAseq\_genes\_rpkm\_20180929.gct.gz dataset?

Thank you very much:)

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**Author:** ![jnoorbak](https://yyz1.discourse-cdn.com/flex035/user_avatar/forum.depmap.org/jnoorbak/32/12_2.png) [@jnoorbak](https://forum.depmap.org/u/jnoorbak)\
**Post date:** [February 12, 2021, 2:30pm UTC](https://forum.depmap.org/t/question-regarding-ccle-rnaseq-dataset/289/2 "2021-02-12T14:30:24Z")

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These are the gene expression data that is used and described in the CCLE paper (Ghandi et al. Nature 2019). The values in that matrix are RPKM values (Reads Per Kilobase of transcript, per Million mapped reads). Typically genes with log2 RPKM \> 0 (or RPKM \>1) could be considered as expressed but for most of the highly expressed genes, the value is much higher.