# Normalization of DepMap CCLE RNAseq Public files **URL:** \ **Category:** Q&A\ **Created:** [August 7, 2022, 11:07pm UTC](https://forum.depmap.org/t/normalization-of-depmap-ccle-rnaseq-public-files/1606 "2022-08-07T23:07:10Z")\ **Posts on this page:** 8\ **Page:** 1
**Author:** ![Chess](https://avatars.discourse-cdn.com/v4/letter/c/c6cbf5/32.png) [@Chess](https://forum.depmap.org/u/Chess)\ **Post date:** [August 7, 2022, 11:07pm UTC](https://forum.depmap.org/t/normalization-of-depmap-ccle-rnaseq-public-files/1606/1 "2022-08-07T23:07:10Z")
I want to use the DepMap Public CCLE RNAseq files. One type of file is CCLE\_RNAseq\_reads.CSV. I understand this to be raw not normalized reads. Is this correct? The other type of file is CCLE\_RNAseq\_transcripts.CSV. I understand this data to be in TPM and to be normalized for read depth and gene length. Is this a correct understanding? Is it valid to compare gene counts from one cell line with another cell line in the CCLE\_RNAseq\_transcripts.CSV data without further normalization? Thanks very much. ---
**Author:** ![jkobject](https://yyz1.discourse-cdn.com/flex035/user_avatar/forum.depmap.org/jkobject/32/82_2.png) [@jkobject](https://forum.depmap.org/u/jkobject)\ **Post date:** [August 22, 2022, 1:25pm UTC](https://forum.depmap.org/t/normalization-of-depmap-ccle-rnaseq-public-files/1606/2 "2022-08-22T13:25:11Z")
Hi Chess, By clicking on the file you should see a documentation pop up. Best, ---
**Author:** ![Pedro\_Sanchez](https://yyz1.discourse-cdn.com/flex035/user_avatar/forum.depmap.org/pedro_sanchez/32/573_2.png) [@Pedro\_Sanchez](https://forum.depmap.org/u/Pedro_Sanchez)\ **Post date:** [August 23, 2022, 3:42pm UTC](https://forum.depmap.org/t/normalization-of-depmap-ccle-rnaseq-public-files/1606/3 "2022-08-23T15:42:32Z")
Hi Jeremie, I am wondering kind of the same. I want to download the data from CCLE\_expression\_transcripts\_expected\_count.csv which are “RNAseq read count data from RSEM”. However, I have not seen an explanation of that in the Nature 2019 paper, since RSEM (version 1.2.22) was only used for quantification of isoform-level expression in TPM (transcripts per million). Do you know how the count data from RSEM were generated or where to find it? I mean, are they, for example, upper quartile normalized RSEM data? Thank you! Pedro ---
**Author:** ![Chess](https://avatars.discourse-cdn.com/v4/letter/c/c6cbf5/32.png) [@Chess](https://forum.depmap.org/u/Chess)\ **Post date:** [August 25, 2022, 9:47am UTC](https://forum.depmap.org/t/normalization-of-depmap-ccle-rnaseq-public-files/1606/4 "2022-08-25T09:47:08Z")
Hi Jkobject, I am confused because when I click on the file, it says gene-level counts were obtained with RSEM; however, in the Ghandi et al. Nature 2019 Supplemental materials (where I think these data came from), it says gene-level counts were obtained as follows “Gene level RPKM and read count values were calculated using RNA-SeQC.” ---
**Author:** ![jkobject](https://yyz1.discourse-cdn.com/flex035/user_avatar/forum.depmap.org/jkobject/32/82_2.png) [@jkobject](https://forum.depmap.org/u/jkobject)\ **Post date:** [August 25, 2022, 1:34pm UTC](https://forum.depmap.org/t/normalization-of-depmap-ccle-rnaseq-public-files/1606/5 "2022-08-25T13:34:32Z")
I think this is where you got confused. The data does not come from the CCLE2 paper (except if you have selected the CCLE 2019 dataset). Since then the pipeline got many iterative updates and the processing is quite different than what was mentioned in the 2019 paper. Hope it helps, ---
**Author:** ![Chess](https://avatars.discourse-cdn.com/v4/letter/c/c6cbf5/32.png) [@Chess](https://forum.depmap.org/u/Chess)\ **Post date:** [August 25, 2022, 9:03pm UTC](https://forum.depmap.org/t/normalization-of-depmap-ccle-rnaseq-public-files/1606/6 "2022-08-25T21:03:10Z")
OK, thanks. ![~WRD0000.jpg](https://canada1.discourse-cdn.com/flex035/uploads/depmap/original/1X/52d5f1c08649212fe6768d2cacdcf8df711810d9.jpeg) ---
**Author:** ![Pedro\_Sanchez](https://yyz1.discourse-cdn.com/flex035/user_avatar/forum.depmap.org/pedro_sanchez/32/573_2.png) [@Pedro\_Sanchez](https://forum.depmap.org/u/Pedro_Sanchez)\ **Post date:** [August 26, 2022, 2:46pm UTC](https://forum.depmap.org/t/normalization-of-depmap-ccle-rnaseq-public-files/1606/7 "2022-08-26T14:46:30Z")
Thanks for the explanation, Jeremie! Can you share then the pipeline in terms of steps you followed to reach those gene-level counts? I am trying to compare these data with others that are upper quantile normalized RSEM and would like to know the way you obtained the CCLE\_expression\_transcripts\_expected\_count.csv Thanks a lot! Pedro ---
**Author:** ![jkobject](https://yyz1.discourse-cdn.com/flex035/user_avatar/forum.depmap.org/jkobject/32/82_2.png) [@jkobject](https://forum.depmap.org/u/jkobject)\ **Post date:** [August 26, 2022, 3:30pm UTC](https://forum.depmap.org/t/normalization-of-depmap-ccle-rnaseq-public-files/1606/8 "2022-08-26T15:30:46Z")
Hi, It is and will always be in > **[GitHub - broadinstitute/depmap\_omics: What you need to process the Quarterly...](https://github.com/broadinstitute/depmap_omics)** > > What you need to process the Quarterly DepMap-Omics releases from Terra - GitHub - broadinstitute/depmap\_omics: What you need to process the Quarterly DepMap-Omics releases from Terra Hope it helps,