# Mutation file, column Tumor\_Seq\_Allele2

**URL:** <https://forum.depmap.org/t/mutation-file-column-tumor-seq-allele2/762>\
**Category:** Q&A\
**Tags:** data\
**Created:** [July 21, 2021, 10:46pm UTC](https://forum.depmap.org/t/mutation-file-column-tumor-seq-allele2/762 "2021-07-21T22:46:16Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![rojinsafavi](https://avatars.discourse-cdn.com/v4/letter/r/ea666f/32.png) [@rojinsafavi](https://forum.depmap.org/u/rojinsafavi)\
**Post date:** [July 21, 2021, 10:46pm UTC](https://forum.depmap.org/t/mutation-file-column-tumor-seq-allele2/762/1 "2021-07-21T22:46:16Z")

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I am not able to find Tumor\_Seq\_Allele2 column in the MAF format of the CCLE data. I was wondering if there is way to get that info?

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**Author:** ![lh12565](https://avatars.discourse-cdn.com/v4/letter/l/d9b06d/32.png) [@lh12565](https://forum.depmap.org/u/lh12565)\
**Post date:** [July 22, 2021, 1:23am UTC](https://forum.depmap.org/t/mutation-file-column-tumor-seq-allele2/762/2 "2021-07-22T01:23:20Z")

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I have the same problem as you.

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**Author:** ![jnoorbak](https://yyz1.discourse-cdn.com/flex035/user_avatar/forum.depmap.org/jnoorbak/32/12_2.png) [@jnoorbak](https://forum.depmap.org/u/jnoorbak)\
**Post date:** [July 22, 2021, 1:42pm UTC](https://forum.depmap.org/t/mutation-file-column-tumor-seq-allele2/762/3 "2021-07-22T13:42:19Z")

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Hi. I believe that a renaming occurred at some point in our datasets and we continued using it to avoid more confusion. So maybe this was an older column name, but there is no `Tumor_Seq_Allele2` in the mutations file. The `Reference_Allele`/`Tumor_Seq_Allele1` columns are the reference/alternate alleles respectively.