# Media Used for KNS-42

**URL:** <https://forum.depmap.org/t/media-used-for-kns-42/281>\
**Category:** Q&A\
**Created:** [November 6, 2020, 8:41pm UTC](https://forum.depmap.org/t/media-used-for-kns-42/281 "2020-11-06T20:41:36Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![S.David](https://avatars.discourse-cdn.com/v4/letter/s/dfb087/32.png) [@S.David](https://forum.depmap.org/u/S.David)\
**Post date:** [November 6, 2020, 8:41pm UTC](https://forum.depmap.org/t/media-used-for-kns-42/281/1 "2020-11-06T20:41:36Z")

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This might be best addressed to someone at the BROAD specifically involved in culturing the KNS-42 line. I was wondering what type of MEM you used. What were the specifics like the concentration of glucose, sodium pyruvate, sodium bicarbonate, and L-glutamine?

Thank you.

S. David

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**Author:** ![pmontgom](https://yyz1.discourse-cdn.com/flex035/user_avatar/forum.depmap.org/pmontgom/32/4_2.png) [@pmontgom](https://forum.depmap.org/u/pmontgom)\
**Post date:** [November 16, 2020, 6:06pm UTC](https://forum.depmap.org/t/media-used-for-kns-42/281/2 "2020-11-16T18:06:30Z")

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The catalog number for the MEM we use is:  
Thermo Fisher cat no: A1451801.

The complete makeup of the medium is 95% MEM + 5% FBS.

Thanks,  
Phil