# Isoform expression data

**URL:** <https://forum.depmap.org/t/isoform-expression-data/2343>\
**Category:** Q&A\
**Created:** [March 2, 2023, 2:23pm UTC](https://forum.depmap.org/t/isoform-expression-data/2343 "2023-03-02T14:23:55Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![Syed\_Haider](https://yyz1.discourse-cdn.com/flex035/user_avatar/forum.depmap.org/syed_haider/32/797_2.png) [@Syed\_Haider](https://forum.depmap.org/u/Syed_Haider)\
**Post date:** [March 2, 2023, 2:23pm UTC](https://forum.depmap.org/t/isoform-expression-data/2343/1 "2023-03-02T14:23:55Z")

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Hi, we are looking at the transcript level data file available in Downloads section:

## OmicsExpressionTranscriptsExpectedCountProfile.csv

What is the scale of these data? Are these cross sample (in this case cell lines) normalised? OR within sample normalised?

Thank you,  
SH

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**Author:** ![simz](https://yyz1.discourse-cdn.com/flex035/user_avatar/forum.depmap.org/simz/32/440_2.png) [@simz](https://forum.depmap.org/u/simz)\
**Post date:** [May 1, 2023, 5:53pm UTC](https://forum.depmap.org/t/isoform-expression-data/2343/2 "2023-05-01T17:53:13Z")

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Hi,

Thanks for reaching out. The expected counts are direct outputs from RSEM’s EM algorithm and are not normalized.

Thanks,  
Simone