# ERCC spike-ins in RNAseq data

**URL:** https://forum.depmap.org/t/ercc-spike-ins-in-rnaseq-data/3606
**Category:** Q&A
**Tags:** data
**Created:** [September 23, 2024, 2:32pm UTC](https://forum.depmap.org/t/ercc-spike-ins-in-rnaseq-data/3606 "2024-09-23T14:32:09Z")
**Posts on this page:** 1
**Page:** 1

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### Author: ![bkinedi](https://avatars.discourse-cdn.com/v4/letter/b/c57346/32.png) [@bkinedi](https://forum.depmap.org/u/bkinedi)
#### Post date: [September 23, 2024, 2:32pm UTC](https://forum.depmap.org/t/ercc-spike-ins-in-rnaseq-data/3606/1 "2024-09-23T14:32:09Z")

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I’m trying to identify differentially expressed genes between two conditions, and would like to use the ERCC spike-ins as control genes when estimating size factors using DESeq2.

I can’t find the annotations for these spike ins and none of the genes or transcripts (`OmicsExpressionTranscriptsExpectedCountProfile` or `OmicsExpressionGeneExpectedCountProfile`) are clearly annotated as ERCC spike ins. Are these filtered prior to publication? Is there any way I can access these?