# Culture medium question about pyruvate

**URL:** <https://forum.depmap.org/t/culture-medium-question-about-pyruvate/4600>\
**Category:** Q&A\
**Tags:** genetic-screens\
**Created:** [March 18, 2026, 2:46pm UTC](https://forum.depmap.org/t/culture-medium-question-about-pyruvate/4600 "2026-03-18T14:46:12Z")\
**Posts on this page:** 4\
**Page:** 1

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**Author:** ![Bimo](https://avatars.discourse-cdn.com/v4/letter/b/f05b48/32.png) [@Bimo](https://forum.depmap.org/u/Bimo)\
**Post date:** [March 18, 2026, 2:46pm UTC](https://forum.depmap.org/t/culture-medium-question-about-pyruvate/4600/1 "2026-03-18T14:46:12Z")

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Dear DepMap Portal,  
I was wondering that if the culture medium is with pyruvate or not, especially the cell line with DMEM. There are mutiple DMEM-high glucose choic from thermo fisher is based on basic DMEM. Our lab prefer 11965, which is not including pyruvate. So I am just curious about this detail, which might influence the experiment outcome.  
Zefang Bimo Zhao

Tsinghua University

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**Author:** ![Bimo](https://avatars.discourse-cdn.com/v4/letter/b/f05b48/32.png) [@Bimo](https://forum.depmap.org/u/Bimo)\
**Post date:** [March 26, 2026, 7:00am UTC](https://forum.depmap.org/t/culture-medium-question-about-pyruvate/4600/2 "2026-03-26T07:00:58Z")

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I really need the answer, especially in the culturation of A549, U2OS, 143B.

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**Author:** ![pmontgom](https://yyz1.discourse-cdn.com/flex035/user_avatar/forum.depmap.org/pmontgom/32/4_2.png) [@pmontgom](https://forum.depmap.org/u/pmontgom)\
**Post date:** [March 30, 2026, 6:59pm UTC](https://forum.depmap.org/t/culture-medium-question-about-pyruvate/4600/3 "2026-03-30T18:59:12Z")

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Hello,

We confirmed our standard DMEM does contain 4.5g/L of sodium pyruvate. We use the Corning media: [https://ecatalog.corning.com/life-sciences/b2c/us/en/Media,-Sera,-and-Reagents/Classical-Media/Dulbecco’s-Modification-of-Eagle’s-Medium-(DMEM)/Corning%C2%AE-DMEM-(Dulbecco%E2%80%99s-Modified-Eagle%E2%80%99s-Medium)/p/15-018-CV](https://ecatalog.corning.com/life-sciences/b2c/us/en/Media,-Sera,-and-Reagents/Classical-Media/Dulbecco's-Modification-of-Eagle's-Medium-(DMEM)/Corning%C2%AE-DMEM-(Dulbecco%E2%80%99s-Modified-Eagle%E2%80%99s-Medium)/p/15-018-CV)

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**Author:** ![Bimo](https://avatars.discourse-cdn.com/v4/letter/b/f05b48/32.png) [@Bimo](https://forum.depmap.org/u/Bimo)\
**Post date:** [March 31, 2026, 2:36am UTC](https://forum.depmap.org/t/culture-medium-question-about-pyruvate/4600/4 "2026-03-31T02:36:12Z")

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Thanks a lot!