# Clarifications in Gene Expression Level csv file

**URL:** <https://forum.depmap.org/t/clarifications-in-gene-expression-level-csv-file/2603>\
**Category:** Q&A\
**Created:** [June 4, 2023, 12:52am UTC](https://forum.depmap.org/t/clarifications-in-gene-expression-level-csv-file/2603 "2023-06-04T00:52:09Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![amenacho](https://yyz1.discourse-cdn.com/flex035/user_avatar/forum.depmap.org/amenacho/32/854_2.png) [@amenacho](https://forum.depmap.org/u/amenacho)\
**Post date:** [June 4, 2023, 12:52am UTC](https://forum.depmap.org/t/clarifications-in-gene-expression-level-csv-file/2603/1 "2023-06-04T00:52:09Z")

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Hi! I just have a question concerning the data in this csv file. I’m trying to calculate the bimodality of each gene but there are some zeros in the entries of the file and I wanted clarity on what they actually mean in terms of the Expression levels. The name of the file is called OmicsExpressionProteinCodingGenesTPMLogp1.csv

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**Author:** ![simz](https://yyz1.discourse-cdn.com/flex035/user_avatar/forum.depmap.org/simz/32/440_2.png) [@simz](https://forum.depmap.org/u/simz)\
**Post date:** [July 19, 2023, 6:02pm UTC](https://forum.depmap.org/t/clarifications-in-gene-expression-level-csv-file/2603/2 "2023-07-19T18:02:46Z")

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Hi,

The values in OmicsExpressionProteinCodingGenesTPMLogp1 are log\_2(TPM-normalized expression values + 1). Therefore, zeros suggest that the gene is not expressed.

Best,  
Simone
