# Cell Line selection based on RPE for func analysis

**URL:** <https://forum.depmap.org/t/cell-line-selection-based-on-rpe-for-func-analysis/2867>\
**Category:** Q&A\
**Created:** [October 16, 2023, 12:56pm UTC](https://forum.depmap.org/t/cell-line-selection-based-on-rpe-for-func-analysis/2867 "2023-10-16T12:56:00Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![rv100](https://avatars.discourse-cdn.com/v4/letter/r/f6c823/32.png) [@rv100](https://forum.depmap.org/u/rv100)\
**Post date:** [October 16, 2023, 12:56pm UTC](https://forum.depmap.org/t/cell-line-selection-based-on-rpe-for-func-analysis/2867/1 "2023-10-16T12:56:00Z")

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Hi, using the Data explorer, and relative protein expression, I checked the genes of interest on X & Y axis and found a couple of cell lines. Since both the genes of interests seem to have positive expression in HCC1143 (primary tumour type) from the attached, is it OK to select HCC1143 cell line to conduct functional assays (knockout)?

Your suggestion will help.Thank you.

 ![DepMap_RPE_Cell Line Selection](https://canada1.discourse-cdn.com/flex035/uploads/depmap/original/1X/4d27f113ec7547f3dec0c8da127e2209d1f18515.png)