# Are these two cell lines same?

**URL:** <https://forum.depmap.org/t/are-these-two-cell-lines-same/1456>\
**Category:** Report an Issue\
**Created:** [June 8, 2022, 5:45pm UTC](https://forum.depmap.org/t/are-these-two-cell-lines-same/1456 "2022-06-08T17:45:13Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![bzhang](https://avatars.discourse-cdn.com/v4/letter/b/258eb7/32.png) [@bzhang](https://forum.depmap.org/u/bzhang)\
**Post date:** [June 8, 2022, 5:45pm UTC](https://forum.depmap.org/t/are-these-two-cell-lines-same/1456/1 "2022-06-08T17:45:13Z")

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I think these two cell lines in CCLE\_gene\_cn.csv from 22Q2 release are same(see below). Would you please check it and let me know. Thanks.

| ACH-000232 | U-251 MG | U251MG | U251MG\_CENTRAL\_NERVOUS\_SYSTEM | U251-MG | 905983 | Male | HSRRB | CVCL\_0021 | 1913 | central\_nervous\_system | Primary | Brain Cancer | Astrocytoma | 75 | SIDM00111 | | central\_nervous\_system | glioma | astrocytoma | | | | | PT-88IC1n | | Astrocytoma | C60781 |
| --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- |
| ACH-001172 | U-251 MG | U251MGDM | U251MGDM\_Brain | | | Male | HSRRB | | | central\_nervous\_system | Primary | Brain Cancer | Astrocytoma | 75 | | | central\_nervous\_system | glioma | astrocytoma | | | | | PT-88IC1n | | | |

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**Author:** ![pmontgom](https://yyz1.discourse-cdn.com/flex035/user_avatar/forum.depmap.org/pmontgom/32/4_2.png) [@pmontgom](https://forum.depmap.org/u/pmontgom)\
**Post date:** [June 27, 2022, 3:28pm UTC](https://forum.depmap.org/t/are-these-two-cell-lines-same/1456/2 "2022-06-27T15:28:33Z")

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Hello,

The relationship between these two lines is explained in the following post:

> [@\[IMPORTANT UPDATE\] Fingerprinting data corrections (21Q3 release)](https://forum.depmap.org/t/important-update-fingerprinting-data-corrections-21q3-release/810):
>
> As part of an ongoing effort to update and improve our data analysis pipelines and release processes, we performed a systematic SNP fingerprinting analysis of all the sequencing data used in DepMap, using the Crosscheck tool ([Javed et al., 2020](https://www.nature.com/articles/s41467-020-17453-5)). This allowed us to test whether our sequencing data for a given cell line were internally consistent, and whether cell lines identified as genetically matched agreed with annotations for derivative/isogenic models. Wherever possible, we also compared ou…

In short, ACH-001172 and ACH-000232 both look like they are U251MG, however, ACH-001172 shows a distinct CN profile suggesting that it has drifted significantly from U251MG. That led us to decide to keep it registered as a distinct cell line separate from ACH-000232.

Thanks,  
Phil