# 21Q1 Combined Achilles + Project Score CRISPR gene effect scores (linear modeling question)

**URL:** <https://forum.depmap.org/t/21q1-combined-achilles-project-score-crispr-gene-effect-scores-linear-modeling-question/446>\
**Category:** Q&A\
**Tags:** data\
**Created:** [February 9, 2021, 4:54pm UTC](https://forum.depmap.org/t/21q1-combined-achilles-project-score-crispr-gene-effect-scores-linear-modeling-question/446 "2021-02-09T16:54:44Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![brodriguez](https://avatars.discourse-cdn.com/v4/letter/b/ed8c4c/32.png) [@brodriguez](https://forum.depmap.org/u/brodriguez)\
**Post date:** [February 9, 2021, 4:54pm UTC](https://forum.depmap.org/t/21q1-combined-achilles-project-score-crispr-gene-effect-scores-linear-modeling-question/446/1 "2021-02-09T16:54:44Z")

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Are the combined score data, scaled on common essential genes, suitable for linear modeling as a response variable?

The ANOVA models produced for Project Score [[publication](https://doi.org/10.1038/s41586-019-1103-9)] identifying CRISPR effect genomic biomarkers were constructed on quantile-normalized, copy-number corrected fold-change values. I am trying to understand whether the gene scaling would create an issue.

Thanks,  
Ben

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**Author:** ![Joshua\_Dempster](https://yyz1.discourse-cdn.com/flex035/user_avatar/forum.depmap.org/joshua_dempster/32/19_2.png) [@Joshua\_Dempster](https://forum.depmap.org/u/Joshua_Dempster)\
**Post date:** [February 9, 2021, 5:46pm UTC](https://forum.depmap.org/t/21q1-combined-achilles-project-score-crispr-gene-effect-scores-linear-modeling-question/446/2 "2021-02-09T17:46:16Z")

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Hi Ben,

The integrated data has been pretty extensively processed to remove batch effects, so a linear model should work fine. Of course no correction method is perfect. If you find anything weird, please let us know.

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**Author:** ![brodriguez](https://avatars.discourse-cdn.com/v4/letter/b/ed8c4c/32.png) [@brodriguez](https://forum.depmap.org/u/brodriguez)\
**Post date:** [February 9, 2021, 6:02pm UTC](https://forum.depmap.org/t/21q1-combined-achilles-project-score-crispr-gene-effect-scores-linear-modeling-question/446/3 "2021-02-09T18:02:46Z")

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Thank you, @Joshua_Dempster .